cfx opus 96 realtime pcr system (Bio-Rad)
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cfx opus 96 realtime pcr system
Cfx Opus 96 Realtime Pcr System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 9499 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cfx+opus+96+realtime+pcr+system/SsoFast+Eva+Green+Supermix/pm41414673-95-36-42
Average 99 stars, based on 9499 article reviews
Cfx Opus 96 Realtime Pcr System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 9499 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cfx+opus+96+realtime+pcr+system/SsoFast+Eva+Green+Supermix/pm41414673-95-36-42
Average 99 stars, based on 9499 article reviews
cfx opus 96 realtime pcr system - by Bioz Stars,
2026-09
99/100 stars
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Quantitative RT-PCR:Article Title: Transcriptional module AdERF195-AdIDH1 regulates citrate degradation in kiwifruit during the low temperature storage Article Snippet: Low temperature storage is widely used to extend the storage life of fleshy fruit, which could affect various fruit quality attributes, including fruit acidity, suggesting a potential link between low temperature and organic acid metabolism.. Citrate is a major component controlling fruit acidity, however, the affection of low temperature on its metabolism remained different among species.. In this study, low temperature treatment (0 C, LT) was verified to rapidly reduce organic acid contents, particularly citrate, in ‘Hayward’ kiwifruit. Article Title: Systematic enhancer mapping and functional analysis in zebrafish with optimized CRISPR interference. Article Snippet: The RNA pellet was washed with 75% (v/v) ethanol, resuspended in 20 μl of RNase-free water, and its concentration and quality were measured using a NanoDrop spectrophotometer. .. For qRT-PCR, total RNA of each sample was reversetranscribed using ABScript Neo RT Master Mix (Abclonal, Cat#RK20433) according to the manufacturer’s instructions. qRT-PCR was performed in duplicate using SsoFast Eva Green Supermix (Bio-Rad, Cat#1725201) on a Article Title: Global DNA methylation and miR-126-3p expression in Mexican women with gestational diabetes mellitus: a pilot study. Article Snippet: Gestational diabetes mellitus (GDM) is a type of diabetes that occurs during pregnancy.. It is characterized by high blood glucose levels detected between gestational weeks 24 and 28 in women who previously did not have diabetes [1].. After delivery, blood glucose levels usually return to normal. Polymerase Chain Reaction:Article Title: Transcriptional module AdERF195-AdIDH1 regulates citrate degradation in kiwifruit during the low temperature storage Article Snippet: Low temperature storage is widely used to extend the storage life of fleshy fruit, which could affect various fruit quality attributes, including fruit acidity, suggesting a potential link between low temperature and organic acid metabolism.. Citrate is a major component controlling fruit acidity, however, the affection of low temperature on its metabolism remained different among species.. In this study, low temperature treatment (0 C, LT) was verified to rapidly reduce organic acid contents, particularly citrate, in ‘Hayward’ kiwifruit. Article Title: Systematic enhancer mapping and functional analysis in zebrafish with optimized CRISPR interference. Article Snippet: The RNA pellet was washed with 75% (v/v) ethanol, resuspended in 20 μl of RNase-free water, and its concentration and quality were measured using a NanoDrop spectrophotometer. .. For qRT-PCR, total RNA of each sample was reversetranscribed using ABScript Neo RT Master Mix (Abclonal, Cat#RK20433) according to the manufacturer’s instructions. qRT-PCR was performed in duplicate using SsoFast Eva Green Supermix (Bio-Rad, Cat#1725201) on a Article Title: Fine Mapping and Identification of Causal Alleles at the Ur-11 Locus Controlling Rust Resistance in Common Bean (Phaseolus vulgaris L.) Article Snippet: PACE SNP genotyping was performed with 20ng of high-quality genomic DNA samples from the genotypes using the PACE 2.0 Genotyping Master Mix (Standard ROX − 150 nM, 3CR Bioscience) in the presence of two competitive allele-speci c forward primers and a common, reverse primer in a nal volume of 8μL (Table S1). .. The PCR ampli cation condition was 15 min at 94°C for the hot start activation, 10 cycles of 20s at 94°C, 65°C for 60s (dropping 0.8°C per cycle), then 38 cycles of 20s at 94°C and 60s at 57°C followed by a nal point read of the uorescence for 2 min at 22°C on a Article Title: Global DNA methylation and miR-126-3p expression in Mexican women with gestational diabetes mellitus: a pilot study. Article Snippet: Gestational diabetes mellitus (GDM) is a type of diabetes that occurs during pregnancy.. It is characterized by high blood glucose levels detected between gestational weeks 24 and 28 in women who previously did not have diabetes [1].. After delivery, blood glucose levels usually return to normal. Article Title: High efficiency protocol for platelet derived fibrin gel loaded with mesenchymal stromal cells extracellular vesicles. Article Snippet: After 48 h, chondrocytes were dissolved and RNA extracted with the miRNeasy Kit (Qiagen, Hilden, Germany), following the manufacturer's instruction. .. First-strand cDNAs were synthesized using the iScript cDNA synthesis kit (Bio-Rad Laboratories, CA, USA) and quantifications performed with iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories) in a Article Title: Extrachromosomal circular DNA promotes prostate cancer progression through the FAM84B/CDKN1B/MYC/WWP1 axis. Article Snippet: Total RNA was extracted from the cells using TRIzol reagent (GK20008, Glpbio, Montclair, CA, USA). .. After reverse transcription with the iScript cDNA Synthesis kit (#1708890, Bio-Rad Laboratories), the SsoAdvanced Universal SYBR Green Supermix (#1725270, Bio-Rad Laboratories) was used for qPCR reactions on a Article Title: MED8 is related to tumor immunity and has predictive value in the prognosis and treatment of glioma. Article Snippet: Subsequently, the RNA was reverse transcribed into cDNA by Evo M-MLV RT Premix for qPCR AG11706 (Accurate Biotechnology (Hunan) Co., Ltd, Changsha, China) following the manufacturer’s instructions. .. RT‒qPCR was then performed using the SYBR Green Premix Pro Taq HS qPCR Kit AG11701 (Accurate Biotechnology (Hunan) Co., Ltd, Changsha, China) and the Applied Biosystems Article Title: Streamlined Specimen Purification for Rapid COVID-19 Diagnosis Using Positively Charged Polymer Thin Film-Coated Surfaces and Chamber Digital PCR. Article Snippet: The ongoing coronavirus disease 2019 (COVID-19) pandemic demands rapid and straightforward diagnostic tools to prevent early-stage viral transmission.. Although nasopharyngeal swabs are a widely used patient sample collection method for diagnosing COVID-19, using these samples for diagnosis without RNA extraction increases the risk of obtaining false-positive and -negative results.. Thus, multiple purification steps are necessary, which are time-consuming, generate significant waste, and result in substantial sample loss. Real-time Polymerase Chain Reaction:Article Title: Transcriptional module AdERF195-AdIDH1 regulates citrate degradation in kiwifruit during the low temperature storage Article Snippet: Low temperature storage is widely used to extend the storage life of fleshy fruit, which could affect various fruit quality attributes, including fruit acidity, suggesting a potential link between low temperature and organic acid metabolism.. Citrate is a major component controlling fruit acidity, however, the affection of low temperature on its metabolism remained different among species.. In this study, low temperature treatment (0 C, LT) was verified to rapidly reduce organic acid contents, particularly citrate, in ‘Hayward’ kiwifruit. Article Title: Extrachromosomal circular DNA promotes prostate cancer progression through the FAM84B/CDKN1B/MYC/WWP1 axis. Article Snippet: Total RNA was extracted from the cells using TRIzol reagent (GK20008, Glpbio, Montclair, CA, USA). .. After reverse transcription with the iScript cDNA Synthesis kit (#1708890, Bio-Rad Laboratories), the SsoAdvanced Universal SYBR Green Supermix (#1725270, Bio-Rad Laboratories) was used for qPCR reactions on a Article Title: MED8 is related to tumor immunity and has predictive value in the prognosis and treatment of glioma. Article Snippet: Subsequently, the RNA was reverse transcribed into cDNA by Evo M-MLV RT Premix for qPCR AG11706 (Accurate Biotechnology (Hunan) Co., Ltd, Changsha, China) following the manufacturer’s instructions. .. RT‒qPCR was then performed using the SYBR Green Premix Pro Taq HS qPCR Kit AG11701 (Accurate Biotechnology (Hunan) Co., Ltd, Changsha, China) and the Applied Biosystems Article Title: Streamlined Specimen Purification for Rapid COVID-19 Diagnosis Using Positively Charged Polymer Thin Film-Coated Surfaces and Chamber Digital PCR. Article Snippet: The ongoing coronavirus disease 2019 (COVID-19) pandemic demands rapid and straightforward diagnostic tools to prevent early-stage viral transmission.. Although nasopharyngeal swabs are a widely used patient sample collection method for diagnosing COVID-19, using these samples for diagnosis without RNA extraction increases the risk of obtaining false-positive and -negative results.. Thus, multiple purification steps are necessary, which are time-consuming, generate significant waste, and result in substantial sample loss. Activation Assay:Article Title: Fine Mapping and Identification of Causal Alleles at the Ur-11 Locus Controlling Rust Resistance in Common Bean (Phaseolus vulgaris L.) Article Snippet: PACE SNP genotyping was performed with 20ng of high-quality genomic DNA samples from the genotypes using the PACE 2.0 Genotyping Master Mix (Standard ROX − 150 nM, 3CR Bioscience) in the presence of two competitive allele-speci c forward primers and a common, reverse primer in a nal volume of 8μL (Table S1). .. The PCR ampli cation condition was 15 min at 94°C for the hot start activation, 10 cycles of 20s at 94°C, 65°C for 60s (dropping 0.8°C per cycle), then 38 cycles of 20s at 94°C and 60s at 57°C followed by a nal point read of the uorescence for 2 min at 22°C on a Maestro Software:Article Title: Fine Mapping and Identification of Causal Alleles at the Ur-11 Locus Controlling Rust Resistance in Common Bean (Phaseolus vulgaris L.) Article Snippet: PACE SNP genotyping was performed with 20ng of high-quality genomic DNA samples from the genotypes using the PACE 2.0 Genotyping Master Mix (Standard ROX − 150 nM, 3CR Bioscience) in the presence of two competitive allele-speci c forward primers and a common, reverse primer in a nal volume of 8μL (Table S1). .. The PCR ampli cation condition was 15 min at 94°C for the hot start activation, 10 cycles of 20s at 94°C, 65°C for 60s (dropping 0.8°C per cycle), then 38 cycles of 20s at 94°C and 60s at 57°C followed by a nal point read of the uorescence for 2 min at 22°C on a Expressing:Article Title: Global DNA methylation and miR-126-3p expression in Mexican women with gestational diabetes mellitus: a pilot study. Article Snippet: Gestational diabetes mellitus (GDM) is a type of diabetes that occurs during pregnancy.. It is characterized by high blood glucose levels detected between gestational weeks 24 and 28 in women who previously did not have diabetes [1].. After delivery, blood glucose levels usually return to normal. Control:Article Title: Global DNA methylation and miR-126-3p expression in Mexican women with gestational diabetes mellitus: a pilot study. Article Snippet: Gestational diabetes mellitus (GDM) is a type of diabetes that occurs during pregnancy.. It is characterized by high blood glucose levels detected between gestational weeks 24 and 28 in women who previously did not have diabetes [1].. After delivery, blood glucose levels usually return to normal. Synthesized:Article Title: High efficiency protocol for platelet derived fibrin gel loaded with mesenchymal stromal cells extracellular vesicles. Article Snippet: After 48 h, chondrocytes were dissolved and RNA extracted with the miRNeasy Kit (Qiagen, Hilden, Germany), following the manufacturer's instruction. .. First-strand cDNAs were synthesized using the iScript cDNA synthesis kit (Bio-Rad Laboratories, CA, USA) and quantifications performed with iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories) in a cDNA Synthesis:Article Title: High efficiency protocol for platelet derived fibrin gel loaded with mesenchymal stromal cells extracellular vesicles. Article Snippet: After 48 h, chondrocytes were dissolved and RNA extracted with the miRNeasy Kit (Qiagen, Hilden, Germany), following the manufacturer's instruction. .. First-strand cDNAs were synthesized using the iScript cDNA synthesis kit (Bio-Rad Laboratories, CA, USA) and quantifications performed with iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories) in a Article Title: Extrachromosomal circular DNA promotes prostate cancer progression through the FAM84B/CDKN1B/MYC/WWP1 axis. Article Snippet: Total RNA was extracted from the cells using TRIzol reagent (GK20008, Glpbio, Montclair, CA, USA). .. After reverse transcription with the iScript cDNA Synthesis kit (#1708890, Bio-Rad Laboratories), the SsoAdvanced Universal SYBR Green Supermix (#1725270, Bio-Rad Laboratories) was used for qPCR reactions on a SYBR Green Assay:Article Title: High efficiency protocol for platelet derived fibrin gel loaded with mesenchymal stromal cells extracellular vesicles. Article Snippet: After 48 h, chondrocytes were dissolved and RNA extracted with the miRNeasy Kit (Qiagen, Hilden, Germany), following the manufacturer's instruction. .. First-strand cDNAs were synthesized using the iScript cDNA synthesis kit (Bio-Rad Laboratories, CA, USA) and quantifications performed with iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories) in a Article Title: Extrachromosomal circular DNA promotes prostate cancer progression through the FAM84B/CDKN1B/MYC/WWP1 axis. Article Snippet: Total RNA was extracted from the cells using TRIzol reagent (GK20008, Glpbio, Montclair, CA, USA). .. After reverse transcription with the iScript cDNA Synthesis kit (#1708890, Bio-Rad Laboratories), the SsoAdvanced Universal SYBR Green Supermix (#1725270, Bio-Rad Laboratories) was used for qPCR reactions on a Article Title: MED8 is related to tumor immunity and has predictive value in the prognosis and treatment of glioma. Article Snippet: Subsequently, the RNA was reverse transcribed into cDNA by Evo M-MLV RT Premix for qPCR AG11706 (Accurate Biotechnology (Hunan) Co., Ltd, Changsha, China) following the manufacturer’s instructions. .. RT‒qPCR was then performed using the SYBR Green Premix Pro Taq HS qPCR Kit AG11701 (Accurate Biotechnology (Hunan) Co., Ltd, Changsha, China) and the Applied Biosystems Reverse Transcription:Article Title: Extrachromosomal circular DNA promotes prostate cancer progression through the FAM84B/CDKN1B/MYC/WWP1 axis. Article Snippet: Total RNA was extracted from the cells using TRIzol reagent (GK20008, Glpbio, Montclair, CA, USA). .. After reverse transcription with the iScript cDNA Synthesis kit (#1708890, Bio-Rad Laboratories), the SsoAdvanced Universal SYBR Green Supermix (#1725270, Bio-Rad Laboratories) was used for qPCR reactions on a |